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ha tagged map3ks  (Addgene inc)
85
Addgene inc ha tagged map3ks
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Ha Tagged Map3ks, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/bio_rxiv__64898__2026__03__09__710604-214-13-24
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anti mekk2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti mekk2
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Anti Mekk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mekk2 - by Bioz Stars, 2026-09
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tri methyl lysine a260 mekk2 antibody  (Epizyme Inc)
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Epizyme Inc tri methyl lysine a260 mekk2 antibody
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Tri Methyl Lysine A260 Mekk2 Antibody, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/a260+antibody+lysine+mekk2+methyl+tri/pm26985287__ml5b00272_si_001-140-0-10
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hatagged mekk2 plasmid dna  (Thermo Fisher)
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Thermo Fisher hatagged mekk2 plasmid dna
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Hatagged Mekk2 Plasmid Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/DNA/pm32071679__ml9b00493_si_001-39-9-26
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hatagged mekk2 plasmid dna - by Bioz Stars, 2026-09
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recombinant kinase proteins mekk2, recombinant, active  (ProQinase GmbH)
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A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Recombinant Kinase Proteins Mekk2, Recombinant, Active, supplied by ProQinase GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/recombinant+kinase+proteins+mekk2/us12264158-913-1-10
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recombinant kinase proteins mekk2, recombinant, active - by Bioz Stars, 2026-09
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mekk2 55106 1 ap  (Proteintech)
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Proteintech mekk2 55106 1 ap
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Mekk2 55106 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/MEKK2+Antibody/pmc11785928__jci___135___173994___s152-12-16-28
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anti mek  (Proteintech)
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Proteintech anti mek
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Anti Mek, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/MEKK2+Antibody/pm39028451-37-19-34
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rabbit anti mekk2  (Danaher Inc)
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Danaher Inc rabbit anti mekk2
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Rabbit Anti Mekk2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/pm38814079-235-42-44
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map3k2 rabbit mab (mekk2, r24953, zenbio) antibody  (ZenBio)
90
ZenBio map3k2 rabbit mab (mekk2, r24953, zenbio) antibody
A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
Map3k2 Rabbit Mab (Mekk2, R24953, Zenbio) Antibody, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mekk2/map3k2+rabbit+mab++mekk2++r24953++zenbio++antibody/pmc11010895-140-10-12
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Image Search Results


A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.

Journal: bioRxiv

Article Title: Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death

doi: 10.64898/2026.03.09.710604

Figure Lengend Snippet: A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.

Article Snippet: Expression constructs encoding N-terminally Venus-tagged arrestin-3 and HA-ASK1 were described earlier ( ); HA-tagged MAP3Ks (ZAKα, ZAKβ, MEKK1, MEKK2, MEKK4, TAK1) were obtained from Addgene (#12182, 12187, 21632, 44160, 141193, 141195, respectively).

Techniques: Transfection, Western Blot, Activation Assay, Control, Binding Assay, Expressing

A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.

Journal: bioRxiv

Article Title: Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death

doi: 10.64898/2026.03.09.710604

Figure Lengend Snippet: A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.

Article Snippet: Expression constructs encoding N-terminally Venus-tagged arrestin-3 and HA-ASK1 were described earlier ( ); HA-tagged MAP3Ks (ZAKα, ZAKβ, MEKK1, MEKK2, MEKK4, TAK1) were obtained from Addgene (#12182, 12187, 21632, 44160, 141193, 141195, respectively).

Techniques: Sequencing, Derivative Assay, Transfection, Control, Activation Assay, Immunoprecipitation, Binding Assay